ABSTRACT
Cells in two-dimensional(2D)cultures gradually lose their original traits as they are passaged in vitro. Existing animal models are expensive and animal experiments require too much work to conduct large-scale experiments. Three-dimensional(3D)cell culture models have attracted increasing attention as they can circumvent the limitations of these previous two models. In 2014, it was reported that the incidence of Alzheimer's disease(AD)in people over 65 years old was 5.14%. In China,the number of AD patients reached 8 million in 2016,but the pathogenesis of the disease is not yet clear. Study of AD is a 'hot' yet complicated issue. This article will briefly introduce 2D and 3D cell cultures and the application of 3D cell culture to AD research in recent years.